Glucagon receptor binding and its effect on the cAMP level in isolated rat and chicken hepatocytes

The binding sites to glucagon with KD 10(-9) M in rat and chicken isolated hepatocytes and with KD 10(-7) M in chicken hepatocytes only where revealed. The first, but not the second binding sites where decreased in their affinity by the adding of GTP nonhydrolyzing analogue Gpp(NH). Glucagon activat...

Full description

Saved in:
Bibliographic Details
Published inFiziologicheskii zhurnal SSSR imeni I. M. Sechenova Vol. 76; no. 9; p. 1153
Main Authors Leĭbush, B N, Ukhanova, M V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1990
Subjects
Animals
Cells, Cultured - metabolism
Chickens
Cyclic AMP - metabolism
Glucagon - metabolism
GTP-Binding Proteins - metabolism
Iodine Radioisotopes
Liver - cytology
Liver - metabolism
Male
Protein Binding
Rats
Rats, Inbred Strains
Receptors, Gastrointestinal Hormone - metabolism
Receptors, Glucagon
Species Specificity
Online AccessGet more information

Cover

More Information
Summary:The binding sites to glucagon with KD 10(-9) M in rat and chicken isolated hepatocytes and with KD 10(-7) M in chicken hepatocytes only where revealed. The first, but not the second binding sites where decreased in their affinity by the adding of GTP nonhydrolyzing analogue Gpp(NH). Glucagon activated the cAMP accumulation in rat and chicken hepatocytes in dose-depended manner. The dose-response curve being plateau after maximum in rats and decreased in chicken. Glucagon inserted an inhibiting effect when occupying chicken-specific sites of binding with KD 10(-7) M. The nature of these binding sites and mechanism of glucagon inhibiting effect on the cAMP accumulation are discussed.
ISSN:0015-329X