Enzymatic synthesis of acyl peptides containing p-nitroanilides of basic amino acids

A method is suggested for synthesis of acylpeptides, containing arginine or lysine p-nitroanilides at the C-terminus, via the acyl transfer reaction catalyzed by the Bacillus subtilis serine proteinase. Acyl-di- and acyltripeptide ethers with L- and D-amino acids were used as the carboxyl component...

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Bibliographic Details
Published inBioorganicheskaia khimiia Vol. 17; no. 8; p. 1066
Main Authors Voiushina, T L, Terent'eva, E Iu, Pozdnev, V F, Gaĭda, A V, Gololobov, M Iu, Liublinskaia, L A, Stepanov, V M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.08.1991
Subjects
Acylation
Amino Acid Sequence
Amino Acids - chemistry
Bacillus subtilis - enzymology
Catalysis
Dimethylformamide - chemistry
Kinetics
Molecular Sequence Data
Peptides - chemical synthesis
Peptides - chemistry
Serine Endopeptidases - metabolism
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Summary:A method is suggested for synthesis of acylpeptides, containing arginine or lysine p-nitroanilides at the C-terminus, via the acyl transfer reaction catalyzed by the Bacillus subtilis serine proteinase. Acyl-di- and acyltripeptide ethers with L- and D-amino acids were used as the carboxyl component taken in a twofold excess. When the concentration of dimethylformamide increases, the hydrolysis of the initial ether and the reaction product diminishes. Because of the enzyme inactivation by dimethylformamide the latter's optimal concentration is 70-80%.
ISSN:0132-3423