Phenoloxidase in the ontogeny of Drosophila melanogaster

Electrophoretic spectrum of phenoloxidase was studied in 5 wild type and 17 mutant lines of D. melanogaster. Low-molecular alcohols (methanol, ethanol, and isopropanol) proved to be activators of the enzyme. Separate components of the phenoloxidase complex were activated by different agents. Electro...

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Bibliographic Details
Published inOntogenez Vol. 21; no. 3; p. 274
Main Authors Belokon', E M, Chernik, Ia I, Bobak, Ia P, Pisotskaia, D E
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1990
Subjects
1-Propanol - pharmacology
Animals
Catechol Oxidase - analysis
Chromosomes - enzymology
Drosophila melanogaster - enzymology
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Electrophoresis, Polyacrylamide Gel - methods
Enzyme Activation - drug effects
Ethanol - pharmacology
Genes - physiology
Genotype
Larva - enzymology
Methanol - pharmacology
Monophenol Monooxygenase - analysis
Monophenol Monooxygenase - genetics
Mutation
Pupa - enzymology
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Summary:Electrophoretic spectrum of phenoloxidase was studied in 5 wild type and 17 mutant lines of D. melanogaster. Low-molecular alcohols (methanol, ethanol, and isopropanol) proved to be activators of the enzyme. Separate components of the phenoloxidase complex were activated by different agents. Electrophoretic spectrum of phenoloxidase was identical in larvae of 3rd age and 24-hour pupae. It consists of three fractions (A1, A2, and A3). Only the A1 fraction was seen on electrophoregrams of imago. No sex or age differences in phenoloxidase spectrum were observed during 25 days after birth. According to the A1 fraction polymorphism there were both normal and mutant genes coding for the A1 phenoloxidase subunit. The Dox-A1 gene that controls monophenoloxydase is located on the 2nd chromosome and is not linked with Dox-A2 and Dox-A3 genes coding for diphenoloxidases.
ISSN:0475-1450