Certain aspects of transformation of 21-acetate of the Reichstein substance "S" by the culture of Tieghemella orchidis

The transformation of acetate from the Reichstein substance "S" by the culture of Tieghemella orchidis was studied upon a single and fractional addition of the substrate. The effect of streptomycin sulphate, Tween-80, ethanol (steroid solvent) and steroid solution on the respiratory activi...

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Published inPrikladnaja biohimija i mikrobiologija Vol. 12; no. 4; p. 485
Main Authors Bukhar, M I, Vdovina, N V, Mogil'nitskiĭ, G M, Shkidchenko, A N, Koshcheenko, K A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1976
Subjects
Acetates - metabolism
Culture Techniques
Ethanol - pharmacology
Fungi - metabolism
Hydrocortisone - pharmacology
Mucorales - metabolism
Oxygen Consumption
Polysorbates - pharmacology
Streptomycin - pharmacology
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Summary:The transformation of acetate from the Reichstein substance "S" by the culture of Tieghemella orchidis was studied upon a single and fractional addition of the substrate. The effect of streptomycin sulphate, Tween-80, ethanol (steroid solvent) and steroid solution on the respiratory activity of the culture was investigated upon a single and fractional addition of the steroid. During transformation the respiratory activity of the culture decreased--by the end of the process (18-20 hours) it fell 2-2.5-fold upon a single addition of the steroid (1 g/l) and by 20% upon a fractional addition of the steroid (2 g/l). In the latter case the respiratory activity dropped step-by-step if the concentration of the steroid was 1.4-1.6 g/l; simultaneously the transformation activity declined.
ISSN:0555-1099