A highly sensitive method of detecting the structural proteins of hepatitis A virus

A modified technique of protein staining with silver nitrate was employed in electrophoretic analysis of hepatitis A virus structural proteins. The modifications of the original technique, i.e. thorough washing of the gel, increased formaldehyde concentration and a more intensive lighting of the gel...

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Bibliographic Details
Published inLaboratornoe delo no. 12; p. 77
Main Authors Dzagurov, G K, Kazachkov, Iu A, Kusov, Iu Iu, Dzugkoeva, F S
Format Journal Article
LanguageRussian
Published Russia (Federation) 1990
Subjects
Electrophoresis, Polyacrylamide Gel
Hepatovirus - analysis
Staining and Labeling
Viral Structural Proteins - analysis
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Summary:A modified technique of protein staining with silver nitrate was employed in electrophoretic analysis of hepatitis A virus structural proteins. The modifications of the original technique, i.e. thorough washing of the gel, increased formaldehyde concentration and a more intensive lighting of the gel, have elevated the method sensitivity 10-fold permitting the detection of up to 0.1 ng protein. The modification has allowed detection of VP1, VP2, and VP3 structural proteins with molecular masses 33, 29, and 27 kD, respectively, in virus preparations of low concentrations.
ISSN:0023-6748