Separation of the ventral disc proteins of Giardia intestinalis

We used repeat isoelectric focusing to obtain cytoskeleton proteins of Giardia intestinalis with the characteristics of giardins and tubulins of the ventral disk. Their molecular weight and its variants by pH difference ranged between 30 and 45 kDa. Polypeptides with molecular weights between 97 and...

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Published inRevista de investigacion clinica Vol. 49; no. 2; pp. 123 - 128
Main Authors Jiménez-Cardoso, E, Crisóstomo-Vázquez, P, Suárez-Souto, A
Format Journal Article
LanguageSpanish
Published Mexico 01.03.1997
Subjects
Animals
Cytoskeletal Proteins - isolation & purification
Fluorescent Antibody Technique, Indirect
Giardia lamblia - chemistry
Giardia lamblia - growth & development
Giardia lamblia - ultrastructure
Hydrogen-Ion Concentration
Immunoenzyme Techniques
Isoelectric Focusing
Membrane Proteins - isolation & purification
Molecular Weight
Protozoan Proteins - isolation & purification
Tubulin - isolation & purification
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Summary:We used repeat isoelectric focusing to obtain cytoskeleton proteins of Giardia intestinalis with the characteristics of giardins and tubulins of the ventral disk. Their molecular weight and its variants by pH difference ranged between 30 and 45 kDa. Polypeptides with molecular weights between 97 and 115 kDa were also obtained. Our immunocytochemical studies showed that the proteins with high molecular weight were from the membrane of the trophozoites; in a smaller proportion we observed also proteins with molecular weights similar to those of giardins and tubulins.
Bibliography:ObjectType-Article-2
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ISSN:0034-8376