Acid hydrolase activity and cyclic nucleotide contents in the rat heart during myocardial ischemia and postischemic reperfusion

The acid phosphatase and cathepsin D activities and cAMP and cGMP levels in isolated perfused rat heart were investigated during various periods of ischaemic myocardial injury and postischaemic reperfusion. The effect of phosphodiesterase inhibitor--caffeine was also studied. Free acid hydrolases ac...

Full description

Saved in:
Bibliographic Details
Published inBiulleten' eksperimental'noi biologii i meditsiny Vol. 108; no. 7; p. 30
Main Authors Ivanova, T N, Ivanov, A I, Korovkin, B F, Losev, N I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1989
Subjects
Acid Phosphatase - analysis
Animals
Caffeine - pharmacology
Cathepsin D - analysis
Coronary Disease - metabolism
Coronary Disease - pathology
Cyclic AMP - analysis
Cyclic GMP - analysis
Male
Myocardial Reperfusion
Myocardium - analysis
Myocardium - pathology
Rats
Time Factors
Online AccessGet more information

Cover

More Information
Summary:The acid phosphatase and cathepsin D activities and cAMP and cGMP levels in isolated perfused rat heart were investigated during various periods of ischaemic myocardial injury and postischaemic reperfusion. The effect of phosphodiesterase inhibitor--caffeine was also studied. Free acid hydrolases activities and cyclic nucleotide content were increased under 40 and 60 min ischemia and 20 min postischaemic reperfusion. Addition of 50 microM caffeine to perfusion solution after 30 min of ischaemia resulted in increase of cAMP level, cAMP/cGMP ratio, lysosomal bound activities of acid hydrolase and decrease of free acid hydrolase activities. The obtained results suggested that defect in cAMP synthesis might be present in lysosomal membranes labilization in cardiomyocytes injured during ischaemic conditions. Addition of such agents, as caffeine, which increased heart cAMP level, may be effective in lysosomal membranes stabilization under reversible heart ischaemia and reperfusion.
ISSN:0365-9615