Evaluation of usefulness of a new generation of tests for detection in humans of antibodies for 9,12 antigens of Salmonella. I. Occurrence and level of antibodies detected by DOT-ELISA, latex and passive hemagglutination tests

• Published in: Medycyna doświadczalna i mikrobiologia Vol. 45; no. 4; p. 439
• Main Author: Szych, J
• Format: Journal Article
• Language: Polish
• Published: Poland 1993
• Subjects: Adult; Antibodies, Bacterial - analysis; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Gastroenteritis - diagnosis; Hemagglutination Tests; Humans; Immunoblotting; Latex Fixation Tests; Middle Aged; Predictive Value of Tests; Reference Values; Salmonella - immunology; Salmonella Infections - diagnosis
• ISSN: 0025-8601

The study was aimed at evaluation of usefulness of immunoenzymatic Dot-ELISA test and an own latex test (OL) for diagnosis of infections evoked by group D Salmonella in humans with application of passive hemagglutination test (OHS) as a relation test. Samples of blood donors, persons suspected of infection with group D Salmonella and of patients with acute gastroenteritis, were tested. In highest percentage of tested samples of serum derived from blood donors (84.5%), antibodies for 9, 12 antigen with Dot-ELISA were detected in immunoglobulin G class. In class IgM these antibodies were found in 46.5% of tested samples, whereas in IgA class only in 11.9% of samples. By application of passive hemagglutination, antibodies against 9, 12 antigen were detected in 56.8% of serum samples from blood donors and by application of a latex test in 8.7% of samples. Evaluation of antibody level of somatic antigen of group D Salmonella in healthy people formed a basis for determination for each of tests of a borderline serum titer accepted as diagnostically significant for specific antigenic stimulation. It permitted for analysis of variability of differences between the level of antibodies for 9, 12 antigen determined in blood donor serum and in patients from other two groups. By all three tests antibodies for 9, 12 antigen were detected significantly more frequently than in blood donors only in samples from patients suspected of Salmonella D infections. In highest percentage of samples (80.4%) these antibodies were detected by the Dot-ELISA test in IgM and IgG classes, slightly lower in OL (70.7% of samples) and in lowest in OHB (57.4% of samples). In samples of serum from patients with acute gastroenteritis, antibodies against 9, 12 antigen were found significantly more frequently than in blood donors only by the Dot-ELISA test in all classes of immunoglobulins (IgM-87.4%, IgA-56.2% and IgG-62.5% of serum samples).