Simultaneous preparation of merozoites and concentrated schizonts of Plasmodium falciparum

A technique has been described for a simultaneous preparation of merozoites and concentrated schizonts. The merozoites were harvested after high speed centrifugation of either the supernatant of the low speed centrifugation of the Plasmodium culture or the portion floated in physiogel. The concentra...

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Bibliographic Details
Published inSoutheast Asian journal of tropical medicine and public health Vol. 12; no. 4; p. 518
Main Authors Tharavanij, S, Prasertsiriroj, V
Format Journal Article
LanguageEnglish
Published Thailand 01.12.1981
Subjects
Culture Media
Methods
Plasmodium falciparum - isolation & purification
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Summary:A technique has been described for a simultaneous preparation of merozoites and concentrated schizonts. The merozoites were harvested after high speed centrifugation of either the supernatant of the low speed centrifugation of the Plasmodium culture or the portion floated in physiogel. The concentrated schizonts were prepared by physiogel floatation technique followed by differential centrifugation in 50% percoll. An average of 56% of parasitized red cells were obtained, of which 64% were schizonts. Concentration of trophozoites and schizonts was 9.5 fold.
ISSN:0125-1562