The monosomy 7 clone in interphase and metaphase cell population: a combined chromosome and primed in situ labeling study

Loss of a chromosome 7 is associated with a poor prognosis in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Some studies have shown higher frequencies of monosomy 7 (-7) in dividing than nondividing myeloid cells, which might indicate that -7 confers a proliferative advantage on...

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Bibliographic Details
Published inActa haematologica Vol. 97; no. 4; p. 216
Main Authors Pedersen, B, Koch, J, Bendix Hansen, K, Hindkjaer, J, Lindbjerg Andersen, C
Format Journal Article
LanguageEnglish
Published Switzerland 1997
Subjects
Acute Disease
Adolescent
Adult
Aged
Aged, 80 and over
Bone Marrow - pathology
Child, Preschool
Chromosome Aberrations
Chromosomes, Human, Pair 7
Clone Cells - ultrastructure
Female
Humans
In Situ Hybridization
Interphase
Karyotyping
Leukemia - genetics
Leukemia - pathology
Leukemia, Myeloid - genetics
Leukemia, Myeloid - pathology
Male
Metaphase
Middle Aged
Monosomy
Myelodysplastic Syndromes - genetics
Myelodysplastic Syndromes - pathology
Neoplasms, Second Primary - genetics
Neoplasms, Second Primary - pathology
Primary Myelofibrosis - genetics
Primary Myelofibrosis - pathology
Sex Distribution
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Summary:Loss of a chromosome 7 is associated with a poor prognosis in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Some studies have shown higher frequencies of monosomy 7 (-7) in dividing than nondividing myeloid cells, which might indicate that -7 confers a proliferative advantage on the host cell. As other groups have not been able to confirm this, we compared the -7 frequencies in bone marrow metaphases as studied with conventional cytogenetics and in interphase cells using primed in situ (PRINS) labeling. We found significantly higher -7 frequencies in metaphase than in interphase cells irrespective of diagnosis and presence or absence of additional chromosome aberrations. Further, we found a significant correlation between the -7 percentages in resting and dividing cells. Finally, as our material showed a clear male preponderance, Mitelman's Catalog of Chromosome Aberrations in Cancer was searched for -7. Of 815 cases with AML or MDS, 491 (60.3%) were found to be men. To our knowledge, this is the first observation of a clear deviation from the 1:1 sex ratio in -7 patients.
ISSN:0001-5792
1421-9662