The effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin on human polymorphonuclear leucocytes
Suspensions of human polymorphonuclear leucocytes were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations 10(-8), 10(-7), 10(-6) and 10(-5) M. The TCDD provoked a progressive extracellular release of the lysosomal enzyme beta-N-acetylglucosaminidase and the cytoplasmic enzyme...
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Published in | Arhiv za higijenu rada i toksikologiju Vol. 45; no. 2; p. 161 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Croatia
01.06.1994
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Subjects | |
Online Access | Get more information |
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Summary: | Suspensions of human polymorphonuclear leucocytes were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations 10(-8), 10(-7), 10(-6) and 10(-5) M. The TCDD provoked a progressive extracellular release of the lysosomal enzyme beta-N-acetylglucosaminidase and the cytoplasmic enzyme lactate dehydrogenase in a dose-dependent fashion. At all concentrations TCDD was much more effective in releasing beta-N-acetylglucosaminidase than lactate dehydrogenase. Different responses of the two enzymes to TCDD might be explained by differential structural affinities of beta-N-acetylglucosaminidase for the lysosomal membrane and of lactate dehydrogenase for the cytoplasm. It is likely that TCDD affected the solubility of beta-N-acetylglucosaminidase to a higher extent than that of lactate dehydrogenase, which is probably firmly attached to the cytoplasm. |
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ISSN: | 0004-1254 1848-6312 |