Determination of phenobarbital by solid-phase immunoenzyme analysis. Choice of optimal strategy

Two variants (direct and indirect) of enzyme linked immunosorbent assay (ELISA) of phenobarbital are compared. Both techniques were developed on the basis of the same monoclonal antibodies, and horse radish peroxidase was used as the label in both cases. When microtitration plates are used as the so...

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Bibliographic Details
Published inBioorganicheskaia khimiia Vol. 21; no. 8; p. 632
Main Authors Danilova, N P, Bekman, N I, Podymova, N G, Maksutova, A L, Zheleznova, E V, Vasilov, R G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.08.1995
Subjects
Animals
Antibodies, Monoclonal - immunology
Enzyme-Linked Immunosorbent Assay - methods
Horseradish Peroxidase
Mice
Phenobarbital - analysis
Phenobarbital - immunology
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Summary:Two variants (direct and indirect) of enzyme linked immunosorbent assay (ELISA) of phenobarbital are compared. Both techniques were developed on the basis of the same monoclonal antibodies, and horse radish peroxidase was used as the label in both cases. When microtitration plates are used as the solid phase, indirect ELISA, in which phenobarbital of the sample competes with phenobarbital sorbed on plates in the form of a conjugate with protein for the binding with peroxidase-labeled antiphenobarbital antibodies, is preferable. In indirect ELISA, the sample volume was 5 microliters, the time of assay was 40 min, the variability coefficient was < 8%.
ISSN:0132-3423