The production of epidermal growth factor and polypeptides similar to it in the intestinal mucosa of rats in 1,2-dimethylhydrazine-induced carcinogenesis and compensatory regeneration

The production of EGF and EGF-like polypeptides in the normal intestinal mucosa and during 1,2-dimethylhydrazine (DMH)-induced carcinogenesis and postresection regeneration was studied in albino rats using chromatographic separation of acid-ethanol extracts. Fractions after gel filtration on Biogel...

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Bibliographic Details
Published inEksperimentalʹnaja onkologija Vol. 11; no. 4; p. 16
Main Authors Fil'chenko, A A, Garmanchuk, L V, Ivashchenko, Iu D, Bykorez, A I
Format Journal Article
LanguageRussian
Published Ukraine 1989
Subjects
1,2-Dimethylhydrazine
Animals
Carcinogens - toxicity
Chromatography, High Pressure Liquid
Dimethylhydrazines - toxicity
Epidermal Growth Factor - analysis
Epidermal Growth Factor - biosynthesis
Hyperplasia - metabolism
Intestinal Mucosa - analysis
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestinal Neoplasms - analysis
Intestinal Neoplasms - chemically induced
Intestinal Neoplasms - metabolism
Intestinal Neoplasms - surgery
Intestine, Small - analysis
Intestine, Small - metabolism
Intestine, Small - surgery
Male
Methylhydrazines - toxicity
Molecular Weight
Peptide Biosynthesis
Peptides - analysis
Rats
Regeneration - drug effects
Time Factors
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Summary:The production of EGF and EGF-like polypeptides in the normal intestinal mucosa and during 1,2-dimethylhydrazine (DMH)-induced carcinogenesis and postresection regeneration was studied in albino rats using chromatographic separation of acid-ethanol extracts. Fractions after gel filtration on Biogel P-60 with subsequent reverse-phase high performance liquid chromatography in acetonitrile gradient were tested in radioreceptor assay for competition with EGF. It has been established that intestinal tumours induced by DMH and regenerating intestinal mucosa have amplified production of EGF--alpha-TGF and related proteins of high molecular weight (approx. 30; 45-55; 120 kD) with the EGF-competitive activity. It is supposed that the high molecular weight EGF-competitive material represents nonprocessed forms of EGF and/or alpha-TGF which are accumulated in rapidly proliferating low-differentiated cells due to the intensified expression of their genes.
ISSN:0204-3564