Evaluation of a mouse anti-IgG-fluorescein conjugate using indirect immunofluorescence and flow cytometry techniques

• Published in: Revista cubana de medicina tropical Vol. 49; no. 2; pp. 120 - 124
• Main Authors: Vilaseca, J C, Pérez, L, Savón, C, Chacón, D, Fernández, S O, Rodríguez, H, Goyenechea, A, Otero, A
• Format: Journal Article
• Language: Spanish
• Published: Cuba 1997
• Subjects: Adult; Animals; Antibodies, Anti-Idiotypic - immunology; Antibodies, Viral - analysis; Antibodies, Viral - immunology; Antibody Specificity; Child; Evaluation Studies as Topic; Flow Cytometry; Fluorescein-5-isothiocyanate; Fluorescent Antibody Technique, Indirect; Fluorescent Dyes; Humans; Indicators and Reagents; Mice; Rabbits; Respiratory Tract Infections - blood; Respiratory Tract Infections - diagnosis; Respiratory Tract Infections - virology; Virus Diseases - blood; Virus Diseases - diagnosis; Virus Diseases - virology
• ISSN: 0375-0760; 1561-3054

An immunoglobulin G of mouse was purified from sera by affinity chromatography in protein A. The rabbits whose sera were able to recognize the antigen injected by double immunodiffusion were immunized with this preparation. The antibodies were precipitated from the rabbit's serum and purified by ion exchange chromatography. This preparation was conjugated to fluorescin isothiocyanate according to the conventional technique. The conjugated obtained was evaluated with the reference strains of Parainfluenza virus 1, 2, 3; Adenovirus; respiratory syncytial virus; and influenza virus A and B, by an indirect immunofluorescence technique and HIV positive samples by flow citometry. Specific monoclonal antibodies were used in both cases. Clinical specimens of patients with acute respiratory infection were evaluated.