Fibronectin-synthesizing activity of free and membrane-bound polyribosomes from human fibroblasts and chick embryos

• Published in: Biokhimiia (Moscow, Russia) Vol. 50; no. 12; p. 2003
• Main Authors: Belkin, V M, Volodarskaia, S M, Mazurov, V I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.12.1985
• Subjects: Animals; Cell-Free System; Cells, Cultured; Chick Embryo; Electrophoresis, Polyacrylamide Gel; Embryo, Mammalian; Fibroblasts - metabolism; Fibronectins - biosynthesis; Humans; Intracellular Membranes - metabolism; Molecular Weight; Peptide Biosynthesis; Polyribosomes - metabolism; Precipitin Tests; Spermidine - pharmacology

Using immunochemical methods, the fibronectin-synthesizing activity of membrane-bound and free polyribosomes in a cell-free system was studied. It was demonstrated that fibronectin biosynthesis on membrane-bound polyribosomes from human embryonic fibroblasts makes up to 4.9%, while that from chicken...