Fibronectin-synthesizing activity of free and membrane-bound polyribosomes from human fibroblasts and chick embryos

Using immunochemical methods, the fibronectin-synthesizing activity of membrane-bound and free polyribosomes in a cell-free system was studied. It was demonstrated that fibronectin biosynthesis on membrane-bound polyribosomes from human embryonic fibroblasts makes up to 4.9%, while that from chicken...

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 50; no. 12; p. 2003
Main Authors Belkin, V M, Volodarskaia, S M, Mazurov, V I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.12.1985
Subjects
Animals
Cell-Free System
Cells, Cultured
Chick Embryo
Electrophoresis, Polyacrylamide Gel
Embryo, Mammalian
Fibroblasts - metabolism
Fibronectins - biosynthesis
Humans
Intracellular Membranes - metabolism
Molecular Weight
Peptide Biosynthesis
Polyribosomes - metabolism
Precipitin Tests
Spermidine - pharmacology
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Summary:Using immunochemical methods, the fibronectin-synthesizing activity of membrane-bound and free polyribosomes in a cell-free system was studied. It was demonstrated that fibronectin biosynthesis on membrane-bound polyribosomes from human embryonic fibroblasts makes up to 4.9%, while that from chicken embryos--1.1% of the total amount of the de novo synthesized proteins (as compared to 1.0 and 0.3% in free polyribosomes, respectively). Fibronectin monomers (Mr = 330 kD) were detected only in the newly synthesized (in the presence of spermidine) material of the cell-free system containing heavy fractions of membrane-bound polyribosomes.
ISSN:0320-9725