Isolation and characteristics of a plasmid from the thermotolerant strain of Bacillus licheniformis
A 8.3 kb cryptic plasmid was isolated from the thermotolerant strain of Bacillus licheniformis 28KA and designated pLT83. The replicative (rep) region was localized on the plasmid map. The pLT83 plasmid labelled in vitro with an antibiotic resistance determinant is able to replicate in B. subtilis c...
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Published in | Molekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 12; p. 16 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Russian |
Published |
Russia (Federation)
01.12.1987
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Subjects | |
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Abstract | A 8.3 kb cryptic plasmid was isolated from the thermotolerant strain of Bacillus licheniformis 28KA and designated pLT83. The replicative (rep) region was localized on the plasmid map. The pLT83 plasmid labelled in vitro with an antibiotic resistance determinant is able to replicate in B. subtilis cells. The pLT83 plasmid replicates stably in B. licheniformis strain at higher temperatures (37-60 degrees C) than in B. subtilis cells (37-50 degrees C). The plasmid and its derivatives may be used as vectors for gene cloning in B. subtilis and B. licheniformis cells. |
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AbstractList | A 8.3 kb cryptic plasmid was isolated from the thermotolerant strain of Bacillus licheniformis 28KA and designated pLT83. The replicative (rep) region was localized on the plasmid map. The pLT83 plasmid labelled in vitro with an antibiotic resistance determinant is able to replicate in B. subtilis cells. The pLT83 plasmid replicates stably in B. licheniformis strain at higher temperatures (37-60 degrees C) than in B. subtilis cells (37-50 degrees C). The plasmid and its derivatives may be used as vectors for gene cloning in B. subtilis and B. licheniformis cells. |
Author | Zhdanov, V G Zvenigorodskiĭ, V I Gaĭdenko, T A Khaĭkinson, M Ia Stepanov, A I |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2833694$$D View this record in MEDLINE/PubMed |
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PublicationTitle | Molekulârnaâ genetika, mikrobiologiâ i virusologiâ |
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Snippet | A 8.3 kb cryptic plasmid was isolated from the thermotolerant strain of Bacillus licheniformis 28KA and designated pLT83. The replicative (rep) region was... |
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SubjectTerms | Bacillus - genetics Bacillus - growth & development DNA Replication DNA Restriction Enzymes DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Genetic Vectors Hot Temperature Plasmids |
Title | Isolation and characteristics of a plasmid from the thermotolerant strain of Bacillus licheniformis |
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