Isolation and characteristics of a plasmid from the thermotolerant strain of Bacillus licheniformis

A 8.3 kb cryptic plasmid was isolated from the thermotolerant strain of Bacillus licheniformis 28KA and designated pLT83. The replicative (rep) region was localized on the plasmid map. The pLT83 plasmid labelled in vitro with an antibiotic resistance determinant is able to replicate in B. subtilis c...

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Bibliographic Details
Published inMolekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 12; p. 16
Main Authors Gaĭdenko, T A, Khaĭkinson, M Ia, Zvenigorodskiĭ, V I, Zhdanov, V G, Stepanov, A I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.12.1987
Subjects
Bacillus - genetics
Bacillus - growth & development
DNA Replication
DNA Restriction Enzymes
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Genetic Vectors
Hot Temperature
Plasmids
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Summary:A 8.3 kb cryptic plasmid was isolated from the thermotolerant strain of Bacillus licheniformis 28KA and designated pLT83. The replicative (rep) region was localized on the plasmid map. The pLT83 plasmid labelled in vitro with an antibiotic resistance determinant is able to replicate in B. subtilis cells. The pLT83 plasmid replicates stably in B. licheniformis strain at higher temperatures (37-60 degrees C) than in B. subtilis cells (37-50 degrees C). The plasmid and its derivatives may be used as vectors for gene cloning in B. subtilis and B. licheniformis cells.
ISSN:0208-0613