Structural parameters of charge density, macroion mobility and dissociation constants of the phosphate groups from conductivity measurements of salt free solutions of poly(U) and its salt

Equivalent conductivity (lambda MeP) was studied for the Li+, Na+, K+, Cs+, NH4+ salts of poly(U) and for polyribouridilic acid. Concentration of the salt free solutions of poly(U) ranges from 1 . 10(-4) M-3 . 10(-3) M. Limiting equivalent conductivity (lambda 0 MeP) was obtained based on linear dep...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 17; no. 2; p. 392
Main Authors Kuznetsov, I A, Vorontsova, O V, Fabrichnaia, O B
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1983
Subjects
Cations, Monovalent
Electric Conductivity
Kinetics
Poly U
Salts
Solutions
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Summary:Equivalent conductivity (lambda MeP) was studied for the Li+, Na+, K+, Cs+, NH4+ salts of poly(U) and for polyribouridilic acid. Concentration of the salt free solutions of poly(U) ranges from 1 . 10(-4) M-3 . 10(-3) M. Limiting equivalent conductivity (lambda 0 MeP) was obtained based on linear dependence of lambda MeP on Cp1/2 (where Cp is the concentration of nucleic phosphorus). The lambda 0 MeP values obtained were shown to increase linearly with the increase of the limiting mobility of counterion. These data were used to calculate limiting mobility of macroion (lambda p = 43 Ohm-1 Cm2 equiv-1) and parameter xi = 1.13 which characterizes charge density on a macroion. Linear dependence was shown to take place for poly(U) acid and it's salts in the Ostwald's coordinates; moreover, pK value for the phosphate groups is practically independent of the counterion's nature and equal 1.93 +/- 0.32.
ISSN:0026-8984