Characteristics of Yersinia strains from clinical material and from other sources. I. Selected biochemical properties

• Published in: Medycyna doświadczalna i mikrobiologia Vol. 48; no. 1-2; p. 21
• Main Authors: Szych, J, Cieślik, A
• Format: Journal Article
• Language: Polish
• Published: Poland 1996
• Subjects: Animals; Blood - microbiology; Feces - microbiology; Food Microbiology; Humans; Species Specificity; Yersinia - classification; Yersinia - isolation & purification; Yersinia - metabolism; Yersinia - pathogenicity
• ISSN: 0025-8601

The purpose of the study was the determination of biochemical features of strains belonging to Yersinia genus isolated from clinical material and other sources, and an assessment of the usefulness of certain biochemical tests for the detection of potentially pathogenic Yersinia strains. In all, 110 strains were studied, including 48 from the archives of the National Institute of Hygiene, 38 isolated from food of animal or plant origin, and 24 isolated from blood and faeces of patients. On the ground of the biochemical features the isolated strains were recognized as belonging to 5 species: Y. enterocolitica(83 strains), Y. pseudotuberculosis(12 strains), Y. frederiksenii(7 strains), Y. kristensenii(2 strains) and Y. intermedia(4 strains). Two strains differed in their features from the typical species reveale as yet in Yersinia genus. All strains isolated from clinical material were recognized as Y. enterocolitica, while those isolated from food included also Y. frederiksenii, Y. intermedia and Y. kristensenii. The isolated strains grew well on CIN medium forming characteristic violet-pink colonies with irregular outlines after 24-48 hours of incubation at 25 degrees C or 37 degrees C. The potential pathogenicity was assessed on the basis of the presence of autoagglutination (AA) and absent ability of breaking down of salicin, aesculin and pyrazinamide. Only two strains of Y. enterocolitica 03 isolated from faeces and 5 strains of Y. pseudotuberculosis from the archives had AA ability. Low frequency of AA was explained with possible loss of plasmids conveying virulence as a result of multiple passages of the strains, and the fact that many of them were present in the R phase. All strains of Y. enterocolitica 03 and both Y. enterocolitica 09 strains from the archives could be assumed to be potentially pathogenic for man and animals, while no strain isolated from food showed the set of features which could suggest its possible pathogenicity.