Nature of the fluorescence spectra of the DNA-specific dye "Hoechst 33258" in solvents and in DNA complexes

Fluorescence spectra of the DNA-specific dye Hoehst 33258 are obtained in a number of solvents. These spectra are interpreted as superpositions of monomeric and dimeric fluorescence bands of this compound. We show that abnormal Stokes' shift in the Hoehst fluorescence occurs only for the dimer...

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Bibliographic Details
Published inBiofizika Vol. 36; no. 1; p. 167
Main Authors Umetskaia, V N, Rozanov, Iu M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1991
Subjects
Base Sequence
Bisbenzimidazole - chemistry
DNA - chemistry
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Solvents
Spectrometry, Fluorescence
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Summary:Fluorescence spectra of the DNA-specific dye Hoehst 33258 are obtained in a number of solvents. These spectra are interpreted as superpositions of monomeric and dimeric fluorescence bands of this compound. We show that abnormal Stokes' shift in the Hoehst fluorescence occurs only for the dimer form of this dye. It is suggested that formation of the A--T-specific complex is realized by dimers of the Hoehst 33258 located in the narrow groove of DNA.
ISSN:0006-3029