Circular permutation of DNA molecules of Streptomyces griseus Kr actinophage Pg2

DNA molecules of phage Pg2 have terminal repetitions and are partially circularly permuted. This was established by denaturation self-reannealing experiments and analysis of the distribution of denaturation loops in partially denatured Pg2 DNA molecules. The genome size is 40.8 kb, terminal repetiti...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 16; no. 1; p. 98
Main Author Sladkova, I A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1982
Subjects
Bacteriophages - analysis
Base Sequence
DNA, Bacterial
DNA, Viral
Microscopy, Electron
Nucleic Acid Conformation
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Repetitive Sequences, Nucleic Acid
Streptomyces griseus - analysis
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Summary:DNA molecules of phage Pg2 have terminal repetitions and are partially circularly permuted. This was established by denaturation self-reannealing experiments and analysis of the distribution of denaturation loops in partially denatured Pg2 DNA molecules. The genome size is 40.8 kb, terminal repetitions are of about 2.2%, the degree of permutation is 35%. On the basis of the data on DNA melting temperature the content of GC pairs was found equal to 65%.
ISSN:0026-8984