Determination of glycemia. Comparison between the enzymatic method and those of Folin-Wu and of ortho-toluidine (author's transl)

On 1032 serum's samples, the determination of glycemia has been at the same time performed with methods enzymatic, of Folin-Wu and of ortho-toluidine. The obtained results, arranged, with the relative frequency, in progressive order from 40 to 470 mg%, have been compared among themselves, takin...

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Published inQuaderni Sclavo di diagnostica clinica e di laboratorio Vol. 12; no. 4; p. 461
Main Author Bersi, S
Format Journal Article
LanguageItalian
Published Italy 01.12.1976
Subjects
Adult
Aged
Autoanalysis
Blood Glucose - analysis
Female
Glucose Oxidase
Humans
Male
Methods
Middle Aged
Molybdenum
Reagent Strips
Toluidines
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Summary:On 1032 serum's samples, the determination of glycemia has been at the same time performed with methods enzymatic, of Folin-Wu and of ortho-toluidine. The obtained results, arranged, with the relative frequency, in progressive order from 40 to 470 mg%, have been compared among themselves, taking as base's value that of the method of Folin-Wu. After a description of the chemical reactions, which occur during the enzymatic determination of the glycemia, and of the causes of error, which can to interfere on the same determination, it has been discussed the validity and the specificity of this method. Effectively this method seems, besides being constantly reproduceable and of simple execution, to consent the best estimation actually possible of the glycemia. It is recommendable a general acceptance of the enzymatic method, executable also with Auto-Analyzer, so that the glycemic values of the same and of the different laboratories could be among themselves comparable.
ISSN:0033-4979