Isolation of cathepsins D by affinity chromatography

The paper deals with the isolation of cathepsin D from rat liver, chicken liver and bovine spleen by affinity chromatography. The synthesis of the adsorbent was performed using the competitive inhibitor of cathepsins D and pepsin pepstatin and activated Sepharose. Application of a pepstatin-Sepharos...

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 40; no. 5; p. 969
Main Authors Kazakova, O V, Orekhovich, V N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1975
Subjects
Animals
Cathepsins - antagonists & inhibitors
Cathepsins - isolation & purification
Cattle
Chickens
Chromatography, Affinity
Liver - enzymology
Pepstatins
Rats
Sepharose
Spleen - enzymology
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Summary:The paper deals with the isolation of cathepsin D from rat liver, chicken liver and bovine spleen by affinity chromatography. The synthesis of the adsorbent was performed using the competitive inhibitor of cathepsins D and pepsin pepstatin and activated Sepharose. Application of a pepstatin-Sepharose column allows obtaining a highly active and purified enzyme in a short period of time.
ISSN:0320-9725