Evidence for the expression of the triosephosphate translocator gene in green and non-green tissue of tomato and potato

• Published in: Plant molecular biology Vol. 31; no. 1; p. 101
• Main Authors: Schünemann, D, Schott, K, Borchert, S, Heldt, H W
• Format: Journal Article
• Language: English
• Published: Netherlands 01.04.1996
• Subjects: Amino Acid Sequence; Base Sequence; Blotting, Northern; Blotting, Western; Carrier Proteins - genetics; Carrier Proteins - metabolism; Chloroplast Proteins; DNA Primers; Glucose-6-Phosphate; Glucosephosphates - metabolism; Hexosephosphates - metabolism; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membrane Transport Proteins; Molecular Sequence Data; Phosphates - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Solanum tuberosum - genetics; Solanum tuberosum - metabolism
• ISSN: 0167-4412

Western blot analysis revealed a cross reaction of an antibody against the spinach triosephosphate translocator with 29 kDa proteins from envelope membranes of plastids from green and red tomato fruits and also of potato tuber amyloplasts. Envelope membranes from potato tubers were isolated from a homogenate of total membranes by isopycnic sucrose density gradient centrifugation. We were able to demonstrate by reverse transcription and sequencing of the PCR product that the mRNA for the triosephosphate translocator in leaves is also present in green and red tomato fruits. The mature protein consists of 330 amino acid residues and is highly homologous to the triosephosphate translocator proteins from potato and tobacco. The PCR product obtained for potato tubers was partly sequenced. It corresponds entirely to the cDNA sequence encoding the potato leaf triosephosphate translocator protein. Evidence for the expression of the triosephosphate translocator gene in various photosynthetic active and inactive tomato tissues (leaf, green fruit, red fruit, root, petal, sepal) and potato tubers was further confirmed by northern blot analysis.