An Sau3 AI restriction endonuclease isoschizomer from Bacillus cereus

The isolation and characterization of a restriction endonuclease from Bacillus cereus IOC 243 are described. The enzyme recognizes the palindromic sequence 5'-G(met-A,A)TC-3' as determined by PEI chromatography of pancreatic DNase, snake venom phosphodiesterase digestion products of labell...

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Bibliographic Details
Published inFEBS letters Vol. 173; no. 1; pp. 99 - 102
Main Authors Cruz, A K, Kidane, G, Pires, M Q, Rabinovitch, L, Guaycurus, T V, Morel, C M
Format Journal Article
LanguageEnglish
Published England 23.07.1984
Subjects
Bacillus cereus - enzymology
Bacteriophage lambda - metabolism
Base Sequence
Deoxyribonucleases, Type II Site-Specific
DNA Restriction Enzymes - isolation & purification
DNA Restriction Enzymes - metabolism
DNA, Viral - metabolism
Substrate Specificity
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Summary:The isolation and characterization of a restriction endonuclease from Bacillus cereus IOC 243 are described. The enzyme recognizes the palindromic sequence 5'-G(met-A,A)TC-3' as determined by PEI chromatography of pancreatic DNase, snake venom phosphodiesterase digestion products of labelled fragments, analysis of restriction digests from normal and N6-methyladenine-free DNA and direct sequence analysis of cloned fragments. The staggered cleavage products with 5' -terminal pGATC extensions are efficiently labelled with polynucleotide kinase and are easily cloned into BamHI sites. The enzyme, denoted Bce243, is thus an isoschizomer of Sau3AI. Its use and potential advantages in substituting Sau3AI. are discussed.
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ISSN:0014-5793