Biological method of blood plasma glucagon determination

Biological method of glucagon determination in the blood plasma was worked out. The method consists in incubation of the rat liver membranes with glucagon which stimulates adenylcyclase, whose activity was determined by the accretion of cAMP content in the incubation medium. Membranes with increased...

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Bibliographic Details
Published inProblemy ėndokrinologii Vol. 26; no. 2; p. 45
Main Authors Kniazeva, A P, Liubarskaia, S G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1980
Subjects
Adenylyl Cyclases - biosynthesis
Animals
Biological Assay - methods
Cell Membrane - metabolism
Cells, Cultured
Cyclic AMP - biosynthesis
Diabetes Mellitus - blood
Glucagon - blood
Humans
In Vitro Techniques
Liver - cytology
Rats
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Summary:Biological method of glucagon determination in the blood plasma was worked out. The method consists in incubation of the rat liver membranes with glucagon which stimulates adenylcyclase, whose activity was determined by the accretion of cAMP content in the incubation medium. Membranes with increased sensitivity of glucagon were obtained; hormone concentrations in the plasma of healthy persons and of patients suffering from diabetes were determined. In healthy persons glucagon content varied from 54 to 175 pg/ml.
ISSN:0375-9660