Design of a chromosomal DNA probe species specific for Yersinia pestis

A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe...

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Bibliographic Details
Published inGenetika Vol. 29; no. 3; p. 417
Main Authors Norkina, O V, Kulichenko, A N, Shovadaeva, G A, Boshnakov, R Kh, Aksenov, M Iu, Popov, Iu A, Tomov, A Ts, K''esovskiĭ, V K, Drozdov, I G, Gintsburg, A L
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1993
Subjects
Base Sequence
Cloning, Molecular
DNA Probes
DNA, Bacterial - genetics
Genes, Bacterial
Molecular Sequence Data
Species Specificity
Yersinia pestis - genetics
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Summary:A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.
ISSN:0016-6758