Isolation and primary characteristics of the common species-specific outer membrane protein of Rickettsia prowazekii

Common species-specific protein is isolated for the first time in the chromatographically pure state from the outer membrane of Rickettsia prowazekii, and its amino acid composition is determined. As revealed by chromatofocusing technique, the protein possesses pI 4.18 +/- 0.03. Three basic ninhydri...

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Bibliographic Details
Published inBioorganicheskaia khimiia Vol. 16; no. 4; p. 457
Main Authors Rodionov, A V, Belousova, L S, Isachkova, I P, Pluzhnikova, G N, Dmitriev, B A, Molokova, E V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.04.1990
Subjects
Amino Acids - analysis
Bacterial Outer Membrane Proteins - analysis
Bacterial Outer Membrane Proteins - isolation & purification
Electrophoresis, Polyacrylamide Gel
Hydrolysis
Molecular Weight
Rickettsia prowazekii - analysis
Species Specificity
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Summary:Common species-specific protein is isolated for the first time in the chromatographically pure state from the outer membrane of Rickettsia prowazekii, and its amino acid composition is determined. As revealed by chromatofocusing technique, the protein possesses pI 4.18 +/- 0.03. Three basic ninhydrin-positive compounds, differing from usual amino acids, were discovered in the protein hydrolyzate. Data suggesting the subunit structure of the isolated protein are presented.
ISSN:0132-3423