The investigation in vivo of the tissue specificity of pig skin fractions

Using an established assay system, a whole aqueous extract of pigskin and the fractions derived from it by ultrafiltration were investigated for their ability to inhibit proliferation in epidermal basal cells of mouse pinna skin in vitro. The fraction between 30KD and 50KD was found to inhibit both...

Full description

Saved in:
Bibliographic Details
Published inVirchows Archiv. B, Cell pathology Vol. 39; no. 1; p. 75
Main Authors Spargo, D J, Laurence, E B
Format Journal Article
LanguageEnglish
Published Germany 1982
Subjects
Animals
DNA - biosynthesis
Growth Inhibitors - analysis
Growth Inhibitors - pharmacology
Mice
Mitosis - drug effects
Skin - analysis
Swine
Tissue Extracts
Online AccessGet more information

Cover

More Information
Summary:Using an established assay system, a whole aqueous extract of pigskin and the fractions derived from it by ultrafiltration were investigated for their ability to inhibit proliferation in epidermal basal cells of mouse pinna skin in vitro. The fraction between 30KD and 50KD was found to inhibit both mitosis and DNA synthesis specifically in epidermal cells. Furthermore, a dose dependent relationship was found between the degree of inhibition of DNA synthesis and the amount of 30-50KD fraction given. The results are discussed in relation to the cytospecific, tissue and organ specific activity of the fractions arising from the assay methods used; furthermore, the implication of interpreting the extent of specificity in this manner is discussed in relation to the definition of the term 'chalone'.
ISSN:0340-6075