Plasmids containing 2 new variants of the E.coli lacZ gene

New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess be...

Full description

Saved in:
Bibliographic Details
Published inBioorganicheskaia khimiia Vol. 14; no. 4; p. 478
Main Authors Khudiakov, Iu E, Kalinina, T I, Nepliueva, V S, Smirnov, V D
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.04.1988
Subjects
Base Sequence
DNA Restriction Enzymes
Escherichia coli - genetics
Lac Operon
Molecular Sequence Data
Plasmids
Online AccessGet more information

Cover

Abstract New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested.
AbstractList New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested.
Author Khudiakov, Iu E
Nepliueva, V S
Smirnov, V D
Kalinina, T I
Author_xml – sequence: 1
  givenname: Iu E
  surname: Khudiakov
  fullname: Khudiakov, Iu E
– sequence: 2
  givenname: T I
  surname: Kalinina
  fullname: Kalinina, T I
– sequence: 3
  givenname: V S
  surname: Nepliueva
  fullname: Nepliueva, V S
– sequence: 4
  givenname: V D
  surname: Smirnov
  fullname: Smirnov, V D
BackLink https://www.ncbi.nlm.nih.gov/pubmed/2844193$$D View this record in MEDLINE/PubMed
BookMark eNotj8FKAzEUAHOo1Fr7CUJ-YCV5L9km3qRULRT00JOX8pJ9rYHd7LJZFf9ewZ7mNszciFnuM8_EQmmECg3gtViVkoLS1nlUBuZiDs4Y7XEhHt5aKl1qiox9nijllM8SZOZv-UVjojwV2Z_k9MFyex_7NsmW4rs8c-ZbcXWitvDqwqU4PG0Pm5dq__q82zzuq8EiVmvvowFHTlvStXJg2XH4C9ERNawJkNBYz7VTFkhbhDoE46AxbHWsCZbi7l87fIaOm-Mwpo7Gn-NlAX4BC19BNQ
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
DocumentTitleAlternate Plazmidy, soderzhashchie dva novykh varianta gena lacZ E. coli
ExternalDocumentID 2844193
Genre English Abstract
Journal Article
GroupedDBID 642
ALMA_UNASSIGNED_HOLDINGS
CGR
CUY
CVF
ECM
EIF
NPM
ID FETCH-LOGICAL-p533-799c428a815a160825e8eb8931c3127a23a3459e68052a15326bb482d4e51c6a2
ISSN 0132-3423
IngestDate Sat Sep 18 07:55:38 EDT 2021
IsPeerReviewed true
IsScholarly true
Issue 4
Language Russian
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-p533-799c428a815a160825e8eb8931c3127a23a3459e68052a15326bb482d4e51c6a2
PMID 2844193
ParticipantIDs pubmed_primary_2844193
PublicationCentury 1900
PublicationDate 1988-Apr
PublicationDateYYYYMMDD 1988-04-01
PublicationDate_xml – month: 04
  year: 1988
  text: 1988-Apr
PublicationDecade 1980
PublicationPlace Russia (Federation)
PublicationPlace_xml – name: Russia (Federation)
PublicationTitle Bioorganicheskaia khimiia
PublicationTitleAlternate Bioorg Khim
PublicationYear 1988
SSID ssib015893042
ssib001786294
ssib001039029
ssib002804347
Score 1.3028876
Snippet New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and...
SourceID pubmed
SourceType Index Database
StartPage 478
SubjectTerms Base Sequence
DNA Restriction Enzymes
Escherichia coli - genetics
Lac Operon
Molecular Sequence Data
Plasmids
Title Plasmids containing 2 new variants of the E.coli lacZ gene
URI https://www.ncbi.nlm.nih.gov/pubmed/2844193
Volume 14
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3PS8MwFMeDPxB2EUWHv8nB26g0adJ03lQmQ3EIVhleJGkzVrZuQ90O_vW-JN1aRUW9lJJAu_aTvbyXvpcvQseJICrgSnhcm9WqQGgv0r72VAKDRcGUzW1V2k0nbN-zqy7vlsp0trrkVZ0kb1_WlfyHKrQBV1Ml-weyi4tCA5wDXzgCYTj-ivEtuL55lr7YhHMn9dCgRiS8MYMQeJ7hYlzLFtx_mDWGMnk0oskf8n_Os7HTdjLaWQOZycagn-VZtjDY1_0pjKLBeGYNyrQsXriWpq7SVZXF5fprR4NnO9Uz2_5QLq7e5dnzyF3locg0Tl0BHrzxMk2lWIIMqGf2DfxgQ1llrLCKQWROoKcCY5JbGjAtMuLEEX_s_LQbdtGzjJZFZMxap7I4Y75qVz8yEgHhWjV6jXwWlNET4eCr-VZeafFMNbRW3OFTmGHdjXgDrRdxAj5z0DfR0vN0C53OgeMSOKYYgOM5cDzuYQCOHXBsgGMDfBvFl634ou0V4hfeBDxwTzSbCUSGMiJcktDE8TrSCn4wSQJChaSBDBhv6tBIUkiYtmioFItoyjQnSShpHa2MxiO9g7DU2hcyZAlPCVM9vxlRFYVhLxUyBRdJ76K6e9Knidvg5Kl4BXvfdeyjWjkyDtBqD_5P-hCcs1d1ZJG8A7FRNBQ
link.rule.ids 783
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Plasmids+containing+2+new+variants+of+the+E.coli+lacZ+gene&rft.jtitle=Bioorganicheskaia+khimiia&rft.au=Khudiakov%2C+Iu+E&rft.au=Kalinina%2C+T+I&rft.au=Nepliueva%2C+V+S&rft.au=Smirnov%2C+V+D&rft.date=1988-04-01&rft.issn=0132-3423&rft.volume=14&rft.issue=4&rft.spage=478&rft_id=info%3Apmid%2F2844193&rft_id=info%3Apmid%2F2844193&rft.externalDocID=2844193
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0132-3423&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0132-3423&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0132-3423&client=summon