Plasmids containing 2 new variants of the E.coli lacZ gene
New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess be...
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Published in | Bioorganicheskaia khimiia Vol. 14; no. 4; p. 478 |
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Main Authors | , , , |
Format | Journal Article |
Language | Russian |
Published |
Russia (Federation)
01.04.1988
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Subjects | |
Online Access | Get more information |
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Summary: | New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested. |
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ISSN: | 0132-3423 |