Plasmids containing 2 new variants of the E.coli lacZ gene

New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess be...

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Bibliographic Details
Published inBioorganicheskaia khimiia Vol. 14; no. 4; p. 478
Main Authors Khudiakov, Iu E, Kalinina, T I, Nepliueva, V S, Smirnov, V D
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.04.1988
Subjects
Base Sequence
DNA Restriction Enzymes
Escherichia coli - genetics
Lac Operon
Molecular Sequence Data
Plasmids
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Summary:New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested.
ISSN:0132-3423