Subcloning and study of the GTP-cyclohydrolase gene of Bacillus subtilis

Bacillus subtilis GTP-cyclohydrolase gene and its deletion derivatives were subcloned in Escherichia coli cells. The position of the gene within the riboflavine operon was defined. The deletion of the 14 kDa fragment from the N-end of GTP-cyclohydrolase gene did not affect the enzyme activity.

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Bibliographic Details
Published inMolekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 7; p. 22
Main Authors Boretskiĭ, Iu R, Drobinskaia, I E, Batchikova, N V, Bidnenko, V E, Rabinovich, P M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1991
Subjects
Bacillus subtilis - enzymology
Cloning, Molecular
DNA, Bacterial - genetics
Electrophoresis
GTP Cyclohydrolase - genetics
Magnesium - metabolism
Manganese - metabolism
Operon
Plasmids
Protein Biosynthesis
Restriction Mapping
Riboflavin - genetics
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Summary:Bacillus subtilis GTP-cyclohydrolase gene and its deletion derivatives were subcloned in Escherichia coli cells. The position of the gene within the riboflavine operon was defined. The deletion of the 14 kDa fragment from the N-end of GTP-cyclohydrolase gene did not affect the enzyme activity.
ISSN:0208-0613