Activation of ras and myc proto-oncogenes in human breast carcinoma and neuroblastoma

DNA from human breast carcinoma (SK-BR-3) and neuroblastoma (LA-N-1) cell lines are capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer. The blot hybridization analysis of DNA from primary and secondary NIH 3T3 transformants identified additional sequences homologo...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 20; no. 5; p. 1236
Main Authors Kniazev, P G, Schafer, R, Willecke, K, Pluzhnikova, G F, Serova, O M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1986
Subjects
Animals
Breast Neoplasms - genetics
Cell Line
DNA Restriction Enzymes
DNA, Neoplasm - genetics
Gene Amplification
Humans
Mice
Neuroblastoma - genetics
Nucleic Acid Hybridization
Proto-Oncogenes
Sequence Homology, Nucleic Acid
Transfection
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Summary:DNA from human breast carcinoma (SK-BR-3) and neuroblastoma (LA-N-1) cell lines are capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer. The blot hybridization analysis of DNA from primary and secondary NIH 3T3 transformants identified additional sequences homologous to the c-Ha-ras 1 oncogene, and revealed amplification of nucleotide sequences homologous to the v-myc oncogene. Restriction fragments of the amplified myc-related sequences correspond to c-myc (SK-BR-3) and N-myc (LA-N-1) loci of the human genome. The results show that active Ha-ras oncogenes can coexist with altered myc oncogenes in breast carcinomas and neuroblastomas. This suggests that a multi-step mechanism involves both ras and myc genes and their cooperation in the development of these tumors.
ISSN:0026-8984