Histologic study of corneal lesions caused by the slime-GLP glycolipoprotein of Pseudomonas aeruginosa

The Pseudomonas aeruginosa slime-glycolipoprotein (GLP) is considered as one of the principal pathogenetical factors of the bacterium. A single dose of 100 micrograms of the P. aeruginosa slime-GLP was injected in rabbit corneas intrastromally. Light microscopy showed that 4 hours after the injectio...

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Published inOphtalmologie (Paris) Vol. 4; no. 1; p. 72
Main Authors Pharmakakis, N, Papadakis, E, Gartaganis, S, Bechrakis, M, Dimitracopoulos, G, Varakis, I
Format Journal Article
LanguageFrench
Published France 01.01.1990
Subjects
Animals
Bacterial Proteins - toxicity
Cornea - drug effects
Cornea - pathology
Glycoproteins - toxicity
Lipoproteins - toxicity
Pseudomonas aeruginosa
Rabbits
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Summary:The Pseudomonas aeruginosa slime-glycolipoprotein (GLP) is considered as one of the principal pathogenetical factors of the bacterium. A single dose of 100 micrograms of the P. aeruginosa slime-GLP was injected in rabbit corneas intrastromally. Light microscopy showed that 4 hours after the injection, polymorphonuclear leucocytes (PMNs) began to infiltrate the anterior stroma. 24 hours after the intrastromal injection, PMNs had infiltrated full corneal thickness followed by multiple absceses formation, loss of epithelial and endothelial cells, disorganisation of normal collagen fibres and hyperplasy of fibroblasts. These morphological observations are very similar to those observed during experimental P. aeruginosa keratitis and show that the P. aeruginosa slime-GLP is at least in part responsible for the characteristic liquefaction necrosis of the keratitis induced by the P. aeruginosa.
ISSN:0989-3105