Concentration and purification of the measles virus using a 2-phase system of water-soluble polymers

A number of two-phase aqueous polymers based on dextran (mol. w. 500) and polyethylene glycol (mol. w. 1000, 4000, 6000) was studied. The two-step distribution of virus-containing tissue culture fluid in these systems was found to result in significant purification of measles virus from protein admi...

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Bibliographic Details
Published inVoprosy virusologiĭ Vol. 30; no. 6; p. 732
Main Authors Pavlenko, I V, Krasheniuk, A I, Sal'nikova, T A, Brezgun, A A, Smorodinova, I P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.11.1985
Subjects
Dextrans - pharmacology
Measles virus - isolation & purification
Methods
Molecular Weight
Polyethylene Glycols - pharmacology
Polymers - pharmacology
Solubility
Viral Proteins - isolation & purification
Virus Cultivation
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Summary:A number of two-phase aqueous polymers based on dextran (mol. w. 500) and polyethylene glycol (mol. w. 1000, 4000, 6000) was studied. The two-step distribution of virus-containing tissue culture fluid in these systems was found to result in significant purification of measles virus from protein admixtures. The degree of virus purification was shown to depend on ratios of volumes and molecular weights of the polymers.
ISSN:0507-4088