Local re-utilization of thymidine in normal mouse tissues as measured with iododeoxyuridine

Thymidine (TdR) and its analogue, iododeoxyuridine (IdUdr), were used to quantitate nucleoside re-utilisation in vivo. Significantly different results obtained, however, depending upon what form of isotopically labelled iododeoxyuridine is used. No measurable local thymidine re-utilization was found...

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Bibliographic Details
Published inCell and tissue kinetics Vol. 21; no. 6; p. 381
Main Authors Quackenbush, R C, Shields, A F
Format Journal Article
LanguageEnglish
Published England 01.11.1988
Subjects
Animals
Bone Marrow - metabolism
Bone Marrow Cells
Carbon Radioisotopes
Cell Division
Idoxuridine
Iodine Radioisotopes
Mice
Mice, Inbred BALB C
Spleen - cytology
Spleen - metabolism
Thymidine - metabolism
Thymus Gland - cytology
Thymus Gland - metabolism
Tritium
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Summary:Thymidine (TdR) and its analogue, iododeoxyuridine (IdUdr), were used to quantitate nucleoside re-utilisation in vivo. Significantly different results obtained, however, depending upon what form of isotopically labelled iododeoxyuridine is used. No measurable local thymidine re-utilization was found in mouse thymus, spleen or bone marrow when the retention of [3H]IdUdR was compared with [14C]TdR. On the other hand, significant differences were found between the retention of [125I]IdUdR and [3H]IdUdR, which is attributed to de-iodination of iododeoxyuridine. Some thymidine re-utilization was found in duodenum using both [3H]IdUdR and [125I]IdUdR. Information on the in vivo distribution of TdR and the contention that a large degree of TdR re-utilization in the thymus is evidence of extensive cell death must be re-interpreted in the light of these results. In addition, evidence for little or no local re-utilization in some tissues will greatly simplify the use of [11C]TdR as an imaging agent for measuring tissue proliferation in vivo with positron emission tomography (PET).
ISSN:0008-8730