Changes in the proteolytic activity of tissues during aging

The intensity of proteolytic processes and qualitative composition of autolysis products of the brain, liver and testicle tissues of young and old rats were studied. The gel-chromatographic analysis (Sephadex G-15 and G-50) revealed no considerable amount of high-molecular peptides (1500 Da and over...

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Bibliographic Details
Published inUkrainskij biohimičeskij žurnal Vol. 62; no. 2; p. 47
Main Authors Goncharov, A Iu, Kizil'shteĭn, A L, Tsybul'skiĭ, I E, Lukash, A I
Format Journal Article
LanguageRussian
Published Ukraine 01.03.1990
Subjects
Aging - metabolism
Amino Acids - metabolism
Animals
Autolysis
Brain - metabolism
Hydrolysis
Liver - metabolism
Male
Peptide Hydrolases - metabolism
Rats
Testis - metabolism
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Summary:The intensity of proteolytic processes and qualitative composition of autolysis products of the brain, liver and testicle tissues of young and old rats were studied. The gel-chromatographic analysis (Sephadex G-15 and G-50) revealed no considerable amount of high-molecular peptides (1500 Da and over) before and after autolysis. The measurement of the quantity of free amino groups in the gel-chromatographic fraction after the complete acid hydrolysis has confirmed that result. The low-molecular peptides and free amino acids, are the main products of the tissue autolysis. The intensity of proteolytic processes, determined by an increase in the amount of amino acids depends on the autolysis duration and age of animals. The total increment of amino acids in the brain and liver tissues of old animals for the first hour of autolysis has been higher by 102 and 219% as compared to young ones. The autolysis of testicles of the young and old animals after the first hour of incubation is characterized by the same intensivity. Such a regularity is not revealed when analyzing the same processes by the Lowry method.
ISSN:0201-8470