The transfusion aspects of allogeneic bone marrow transplantation

• Published in: Terapevtic̆eskii arhiv Vol. 70; no. 7; p. 67
• Main Authors: Fregatova, L M, Volkova, O Ia, Shavva, S A, Platonova, G G, Afanas'ev, B V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 1998
• Subjects: ABO Blood-Group System - immunology; Bone Marrow Transplantation - immunology; Bone Marrow Transplantation - methods; Child; Erythrocyte Transfusion - methods; Hemolysis - immunology; Histocompatibility Testing; HLA Antigens - immunology; Humans; Transplantation, Homologous
• ISSN: 0040-3660

Analysis of transplantations of allogenic bone marrow (BM) for determination of transfusiological problems arising in various types of donor's and recipient's blood ABO incompatibility. 46 allogenic BM transplantations from relative donors are analysed. The following types of ABO incompatibility were identified: significant (12 donor-recipient pairs), insignificant (10 pairs), combined (2 pairs). The proposed methods of BM fractionation provided the yield of nucleated cells in minimal admixture of red cells. This allowed to minimize loss of stem cells at fractionation and to prevent acute hemolysis at infusion of BM suspension in cases of ABO group-different BM allotransplantations. The proposed modification of the "shelf" method enabled effective fractionation of small BM volumes obtained from children-BM donors with high yield of nucleated cells and small admixture of incompatible red cells. Reinfusion of erythrocytic mass obtained at fractionation of donor BM fractionation used as replacement therapy makes allogenic blood transfusion unnecessary in 66% of BM donors.