Antinuclear antibodies: analysis of images and classification of immunofluorescence aspects on nuclei isolated from rat hepatocytes

• Published in: Biomedicine & pharmacotherapy Vol. 38; no. 3; p. 149
• Main Authors: Delbarre, F, Dupoisot, H, Mach, P S, Laoussadi, S, Daury, G, Constans, A, Amor, B, de Vernejoul, P, Albarede, P
• Format: Journal Article
• Language: French
• Published: France 1984
• Subjects: Animals; Antibodies, Antinuclear - analysis; Cell Nucleus - immunology; Fluorescent Antibody Technique; Fourier Analysis; Histocytochemistry; Humans; Liver - immunology; Liver - ultrastructure; Rats; Spectrometry, Fluorescence
• ISSN: 0753-3322

Image analysis and Fourier transformation of the various nuclear immunofluorescence patterns observed while detecting antinuclear antibodies allow an objective and quantitative definition of the fluorescence. They also point out various IF types hidden by the main pattern, without having to dilute the test serum. They make obvious the difference between speckled and reticular patterns, and reveal the existence of intermediate states. The usual nuclear IF patterns (homogeneous, ring, nucleolar, reticulated, speckled and diffuse) may be grouped, according to their photo emission, into nuclear and subnuclear patterns. The first group includes homogeneous, annular and passive nucleolar IF. The second group is composed of speckled, reticulated, mixed, and active nucleolar IF. Alternatively, these aspects may be grouped into three types: homogeneous nuclear IF (homogeneous and ring), heterogeneous nuclear IF (speckled, reticulated and mixed) and nucleolar IF (active or passive). Diffusion can affect or not these aspects and does not apply to a special type or pattern. Image analysis and the study of the image spatial spectrum lead to automated recognition of the IF types, and later on, to the discrimination of antinuclear antibodies.