Separation of urologic tumors cells from Cell Saver blood using a membrane filter. A new method in autotransfusion?

Tumor cells of three urological longterm cell lines have been labelled with 35[S]-Methionin and added to red cell concentrates. Red cell concentrates rich in tumor cells were passed through a cell saver and two special membranfilters under standard conditions. The 35[S]-Methionin labelled tumor cell...

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Bibliographic Details
Published inUrologe. Ausgabe A Vol. 31; no. 3; p. 182
Main Authors Wiesel, M, Güdemann, C, Staehler, G, Bierhaus, A, Martin, E, Hoever, K H
Format Journal Article
LanguageGerman
Published Germany 01.05.1992
Subjects
Blood Component Removal - instrumentation
Blood Component Transfusion - instrumentation
Blood Transfusion, Autologous - instrumentation
Carcinoma, Renal Cell - blood
Carcinoma, Transitional Cell - blood
Cell Line
Cell Separation - instrumentation
Humans
Kidney Neoplasms - blood
Male
Neoplastic Cells, Circulating
Prostatic Neoplasms - blood
Urinary Bladder Neoplasms - blood
Urologic Neoplasms - blood
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Summary:Tumor cells of three urological longterm cell lines have been labelled with 35[S]-Methionin and added to red cell concentrates. Red cell concentrates rich in tumor cells were passed through a cell saver and two special membranfilters under standard conditions. The 35[S]-Methionin labelled tumor cells were detected by liquid scintillation counting. On an average, only 0.027% of the radioactivity was left after passing through the cell saver and the membran filters in the 12 experiments. If investigations in clinical use of cell-saver and membran filter confirm these results, there will be significant consequences in urological tumor surgery by the possibility of transfusing the salvaged autologous blood.
ISSN:0340-2592