Transformation of the yeast Hansenula polymorpha by a hybrid plasmid containing the fragment of host DNA

Spheroplasts of Hansenula polymorpha strain deficient in 2-isopropylmalate dehydrogenase have been shown to be transformed by the DNA of a hybrid plasmid pHRI, carrying the LEU2 gene from S. cerevisiae and 2.0 kilobase HindIII fragment of H. polymorpha genomic DNA. The frequency of transformants has...

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Bibliographic Details
Published inMolekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 5; p. 28
Main Authors Makhina, E N, Nesterova, G F, Grishin, A V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1985
Subjects
DNA, Fungal - genetics
Genetic Markers
Mutation
Pichia - genetics
Plasmids
Saccharomycetales - genetics
Transformation, Genetic
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Summary:Spheroplasts of Hansenula polymorpha strain deficient in 2-isopropylmalate dehydrogenase have been shown to be transformed by the DNA of a hybrid plasmid pHRI, carrying the LEU2 gene from S. cerevisiae and 2.0 kilobase HindIII fragment of H. polymorpha genomic DNA. The frequency of transformants has reached 10(3) per 1 microgram of transforming DNA. Plasmid pHRI is maintained in transformants as an autonomous circular DNA molecule and is inherited by 1-2% fraction of cells from the population growing under the selective conditions. Transformation takes place under the same conditions that are required for spheroplast fusion. Thus, H. polymorpha becomes one more species of yeast susceptible to hybrid plasmid-mediated gene transfer in the process of DNA transformation.
ISSN:0208-0613