Lambda plasmidophages and their properties

Plasmidphage lambda NM::pBR322 has been constructed in vitro and characterized. Under normal conditions the hybrid DNA molecule undergoes a lytic cycle of phage development, whereas in the presence of antibiotic lambda DNA replicates in the cell extrachromosomally as a plasmid. Properties of plasmid...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 19; no. 3; p. 610
Main Authors Mel'nikov, A A, Chernov, A P, Fodor, I I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1985
Subjects
Bacteriophage lambda - genetics
Chromosome Mapping
Cloning, Molecular
DNA Replication
DNA Restriction Enzymes
DNA, Viral - genetics
DNA, Viral - isolation & purification
Electrophoresis, Agar Gel
Escherichia coli - genetics
Genetic Markers
Plasmids
Transduction, Genetic
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Summary:Plasmidphage lambda NM::pBR322 has been constructed in vitro and characterized. Under normal conditions the hybrid DNA molecule undergoes a lytic cycle of phage development, whereas in the presence of antibiotic lambda DNA replicates in the cell extrachromosomally as a plasmid. Properties of plasmidphage lambda NM::pBR322 have been compared with the earlier constructed lambda gt::pMB9. It has been demonstrated that plasmid pMB9 in vivo can be precisely excised from the lambda gt::pMB9.
ISSN:0026-8984