Stereological analysis of phagocytosing cells

Various complexes of phagocytes with adsorbed (I) and entrapped (J) particles are formed during phagocytosis. Method of stereological reconstruction is proposed that allows to demonstrate the actual distribution of these complexes on the basis of morphometric analysis of their ultrathin sections. Th...

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Bibliographic Details
Published inT͡S︡itologii͡a Vol. 27; no. 3; p. 336
Main Authors Shcherbukhin, V V, Khramtsov, A V, Morozov, I A, Senchenkov, E P, Zemskov, V M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1985
Subjects
Absorption
Adsorption
Animals
Candida albicans
Kinetics
Macrophages - immunology
Macrophages - ultrastructure
Mathematics
Mice
Mice, Inbred CBA
Models, Biological
Phagocytosis
Time Factors
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Summary:Various complexes of phagocytes with adsorbed (I) and entrapped (J) particles are formed during phagocytosis. Method of stereological reconstruction is proposed that allows to demonstrate the actual distribution of these complexes on the basis of morphometric analysis of their ultrathin sections. The principle of the method lies on the probability simulation of section distribution for a given distribution of complexes and on the solution of the reverse problem by stepwise determination of the relative quantity of each complex type (from the most complicated to the most simple one, when I = 0 and J = 0). The stereological analysis of phagocytosing murine peritoneal macrophages revealed an absolutely different and more adequate kinetical picture of phagocytosis, as compared to the morphometric data.
ISSN:0041-3771