Polyethylene glycol-modified avidin: a novel agent for the selective extraction of biotinylated immune-complex in an aqueous two-phase system

Chicken avidin was chemically modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine (activated PEG2) to form PEG-avidin. The PEG-avidin, in which 78% of the amino groups were modified, retained 49% of the active biotin-binding sites. The modified avidin was partitioned preferenti...

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Bibliographic Details
Published inJournal of biomaterials science. Polymer ed. Vol. 7; no. 3; p. 289
Main Authors Nishimura, H, Munakata, N, Hayashi, K, Hayakawa, M, Iwamoto, H, Terayama, S, Takahata, Y, Kodera, Y, Tsurui, H, Shirai, T
Format Journal Article
LanguageEnglish
Published England 1995
Subjects
Animals
Antigen-Antibody Complex - immunology
Antigen-Antibody Complex - isolation & purification
Antigens - isolation & purification
Avidin - chemistry
Avidin - isolation & purification
Avidin - metabolism
Binding Sites
Biotin - chemistry
Chickens
Dextrans - chemistry
Drug Delivery Systems
Egg White
Immunoglobulin G - immunology
Immunoglobulin G - metabolism
Mice
Polyethylene Glycols - chemistry
Spectrometry, Fluorescence
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Summary:Chicken avidin was chemically modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine (activated PEG2) to form PEG-avidin. The PEG-avidin, in which 78% of the amino groups were modified, retained 49% of the active biotin-binding sites. The modified avidin was partitioned preferentially into the PEG-phase in an aqueous two-phase system (PEG/dextran). Using PEG-avidin, the immune-complex formed between biotinylated anti-mouse IgG and its antigen IgG (mouse) molecules, was successfully transferred into the PEG-phase in an aqueous two-phase system. This finding leads to the effective isolation of a specific antigen among various kinds of antigens by partitioning with a two-phase system using PEG-avidin.
ISSN:0920-5063