Affinity chromatography of mouse and rat alpha-fetoproteins on immobilized diethylstilbestrol

Alpha-fetoproteins (AFP) from amniotic fluid of mouse and rat demonstrate high affinity and specificity during their binding with immobilized diethylstilbestrol, which allows to isolate these two proteins by one step using the method of affinity chromatography on Sepharose with immobilized diethylst...

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Bibliographic Details
Published inBiulleten' eksperimental'noi biologii i meditsiny Vol. 113; no. 6; p. 588
Main Authors Terent'ev, A A, Moldogazieva, N T, Tatarinov, Iu S
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.06.1992
Subjects
alpha-Fetoproteins - analysis
Amniotic Fluid - chemistry
Animals
Binding Sites
Chromatography, Affinity
Diethylstilbestrol - analysis
Diethylstilbestrol - metabolism
Electrophoresis, Disc
Estradiol - metabolism
Female
Immunodiffusion
Mice
Pregnancy
Rats
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Summary:Alpha-fetoproteins (AFP) from amniotic fluid of mouse and rat demonstrate high affinity and specificity during their binding with immobilized diethylstilbestrol, which allows to isolate these two proteins by one step using the method of affinity chromatography on Sepharose with immobilized diethylstilbestrol. Meanwhile the yield of mouse AFP was 42%, and rat AFP--75%. The preliminary incubation of the amniotic fluid of rat and mouse with free estradiol results in abrupt fall of AFP outcome, which may testify to the binding of estradiol and diethylstilbestrol by the same receptor sites on AFP molecule.
ISSN:0365-9615