Comparative study of the repeated nucleotide sequences in DNA from differentiated tissues and tumors

DNA renaturation kinetics was measured for the genomes of normal (spleen) and malignant (plasmacytoma) mouse tissues and for DNA from liver, sperm and developing embryos of the loach. It has been shown that the measuring of DNA renaturation kinetics makes it possible to reveal differences in the con...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 9; no. 1; p. 126
Main Authors Timofeeva, M Ia, Eĭsner, G I, Kupriianova, N S
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1975
Subjects
Animals
Base Sequence
Chromatin - analysis
DNA
DNA, Neoplasm
Embryo, Nonmammalian
Fishes
Kinetics
Liver - analysis
Male
Mice
Nucleic Acid Renaturation
Organ Specificity
Plasmacytoma - analysis
Spermatozoa - analysis
Spleen - analysis
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Summary:DNA renaturation kinetics was measured for the genomes of normal (spleen) and malignant (plasmacytoma) mouse tissues and for DNA from liver, sperm and developing embryos of the loach. It has been shown that the measuring of DNA renaturation kinetics makes it possible to reveal differences in the content of certain fractions of the repetitions in the genomes of different species. Moreover, differences in the distribution of the repetitions between hetero- and euchromatine can be identified. Loach embryo DNA (blastula stage) was shown to contain larger amount of the fraction renaturing at Cot less than 10(-2) as compared to liver and sperm DNAs (by 5%). An enrichment with respect to the intermediate repetitions (10(-2) less than Cot less than 10(2)) was found in the mouse plasmacytoma DNA as compared to the spleen DNA. The nature of these distinctions is discussed.
ISSN:0026-8984