Cloning of DNA complementary to mRNA for proopiomelanocortin from the bovine, rat and human hypophysis. Hormonal regulation of proopiomelanocortin mRNA in the rat hypophysis

Cloning of DNA and complementary mRNA of bovine, rat and human proopiomelanocortin (POMC) was carried out. A structural analysis of the cloned cDNA of POMC was performed. Using restriction fragments of bovine, rat and human POMC cloned cDNA, probes for molecular hybridization based on one-chain bact...

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Published inBiokhimiia (Moscow, Russia) Vol. 52; no. 5; p. 707
Main Authors Mertvetsov, N P, Golovin, S Ia, Beklemishev, A B, Karginov, V A, Mamaev, L V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1987
Subjects
Adrenalectomy
Animals
Base Sequence
Cattle
Cloning, Molecular
DNA - analysis
Estradiol - physiology
Female
Genetic Markers
Humans
Nucleic Acid Hybridization
Pituitary Gland - analysis
Pro-Opiomelanocortin - genetics
Rats
Rats, Inbred Strains
RNA, Messenger - analysis
RNA, Messenger - genetics
Species Specificity
Transcription, Genetic
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Summary:Cloning of DNA and complementary mRNA of bovine, rat and human proopiomelanocortin (POMC) was carried out. A structural analysis of the cloned cDNA of POMC was performed. Using restriction fragments of bovine, rat and human POMC cloned cDNA, probes for molecular hybridization based on one-chain bacteriophage M13 were made. Using the dot-hybridization technique with labeled [32P] POMC cDNA, the effect of 17 beta-estradiol and adrenalectomy on the POMC mRNA level in rat hypophysis was studied. The hormone was shown to significantly decrease the POMC mRNA content at a concentration of 4 and 8 micrograms per 100 g of body weight 4 hours after injection. Adrenalectomy caused a 2-3-fold increase in the POMC mRNA level in rat hypophysis after a period of 5-7 days. The possibility of polyhormonal control of POMC genome transcription is discussed.
ISSN:0320-9725