Assignment of the human prohibitin gene (PHB) to chromosome 17 and identification of a DNA polymorphism

Prohibition is a recently identified antiproliferative protein whose exact role in the cell is under investigation. To determine the human chromosomal location of the prohibition gene (PHB) and whether this site corresponds to that of any suspected tumor suppressor gene, we have analyzed DNA from th...

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Published inGenomics (San Diego, Calif.) Vol. 11; no. 1; pp. 228 - 230
Main Authors White, J J, Ledbetter, D H, Eddy, Jr, R L, Shows, T B, Stewart, D A, Nuell, M J, Friedman, V, Wood, C M, Owens, G A, McClung, J K
Format Journal Article
LanguageEnglish
Published United States 01.09.1991
Subjects
Blotting, Southern
Chromosome Banding
Chromosome Mapping
Chromosomes, Human, Pair 17
Female
Humans
Male
Pedigree
Polymorphism, Restriction Fragment Length
Proteins - genetics
Repressor Proteins
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Summary:Prohibition is a recently identified antiproliferative protein whose exact role in the cell is under investigation. To determine the human chromosomal location of the prohibition gene (PHB) and whether this site corresponds to that of any suspected tumor suppressor gene, we have analyzed DNA from three sources by hybridization analysis: mouse--human hybrid cell lines, hybrid cell lines containing portions of human chromosomes, and human metaphase chromosomes in situ. All three techniques confirm a location in the region 17q21-q22, a region genetically linked to early-onset human breast cancer. Further analysis will be required to establish the significance of this relationship; Southern hybridizations show a polymorphic EcoRI site that may be useful for this purpose.
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ISSN:0888-7543