Selective method of isolating mutants sensitive to ultraviolet radiation from a culture of Rhodopseudomonas sphaeroides

The method of penicillin selection used after UV-irradiation (lambda = 254 nm) allows one to select UV-sensitive mutants (uvs-mutants) of the phototrophous bacterium Rhodopseudomonas sphaeroides induced by nitrosomethylurea with an effectiveness greater by an order of magnitude. Over 30% of the uvs-...

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Bibliographic Details
Published inMikrobiologija (Moskva. 1932) Vol. 55; no. 3; p. 391
Main Authors Rodina, N E, Kameneva, S V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1986
Subjects
Genetic Techniques
Methylnitrosourea - pharmacology
Mutation
Penicillins - pharmacology
Radiation Tolerance
Rhodobacter sphaeroides - drug effects
Rhodobacter sphaeroides - isolation & purification
Rhodobacter sphaeroides - radiation effects
Selection, Genetic
Ultraviolet Rays
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Summary:The method of penicillin selection used after UV-irradiation (lambda = 254 nm) allows one to select UV-sensitive mutants (uvs-mutants) of the phototrophous bacterium Rhodopseudomonas sphaeroides induced by nitrosomethylurea with an effectiveness greater by an order of magnitude. Over 30% of the uvs-mutants obtained using this method had an elevated sensitivity not only to far-UV (F-UV, lambda = 254 nm) but also to near-UV (N-UV, lambda greater than 280 nm) UV-irradiation. No correlation was found in the degree of sensitivity to F-UV and N-UV-irradiation of the uvs-mutants. Mutants highly sensitive to the lethal action of N-UV were isolated.
ISSN:0026-3656